OsmoTECH® Single-Sample Micro-Osmometer

OsmoTECH^® Single-Sample Micro-Osmometer

Basic osmolality testing to meet your needs

Measure osmolality of your liquid samples
Small sample requirement
Essential data integrity and management features

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This [OsmoTECH] instrument was a dream come true. The administrator can lock down features and dictate what users can and cannot do. Very easy to use and extremely reliable instrument.

Troy Franz / Paragon Bioservices

Single-sample osmometer
Basic data management capability
Essential data integrity features
Supports Pharmacopeia osmolality testing guidelines

Ensure quality in your process

The OsmoTECH single-sample osmometer is an accurate, precise, and easy to use instrument built with the freezing point technology. The OsmoTECH is enhanced with data management options and data compliance features to support research and startups.

Industry Applications

In Biotech Labs

Maximize biologic yield, quality, and purity by optimizing and ensuring the quality and consistency of your buffers, media, product, excipients, and process parameters.

In Food & Beverage Labs

Maintain efficiency while ensuring product quality and process consistency with osmolality testing from formulation to release.

Product Features

Menu-driven operating system
Multi-language capability
Touchscreen graphic interface

Only 20 µL of sample

Integrated barcode scanner
Basic data management

Active Directory
Automatic or manual backups
Multi-level user access

Save data to network database, USB drive, or embedded Web server

Open Protocol Communication (OPC)
USB download
Network share
IS (TCP/IP communication)
Optional DOT-MATRIX PRINTER

Sample type	Aqueous-based solution
Sample volume	20 ± 1 μL
Test time	90 seconds
Sample capacity	Single sample
Units	mOsm/kg H₂O
Resolution	1 mOsm/kg H₂O
Range	0-2000 mOsm/kg H₂O
Accuracy²	0-400 mOsm/kg H₂O: mean value ± 2 mOsm/kg H₂O from nominal value (1 SD) >400- to <1500 mOsm/kg H₂O:≤ 0.5% from nominal value (1 SD) >1500- to 2000 mOsm/kg H₂O:≤ 1% from nominal value (1 SD)
Within-run repeatability²	0-400 mOsm/kg H₂O: standard deviation ≤ 2 mOsm/kg H₂O >400 to <1500 mOsm/kg H₂O: Coefficient of Variation ≤ 0.5% >1500 to <2000 mOsm/kg H₂O: Coefficient of Variation ≤ 1%
Temperature effects³	< 1 mOsm/kg H₂O per 5°C (9°F) ambient temperature change
Calibration	0, 50, 850, and 2000 mOsm/kg H2O (* optional)
Communication	USB 2.0 Type A Port (2), USB 2.0 Type A Port (1), Ethernet 10/100, RJ45 connector port (1) dot matrix printer (optional)
Supported languages	Simple Chinese, Czech, Danish, Greek, Portuguese, English, French, German, Italian, Japanese, Korean, Russian, Spanish, Turkish
Storage temperature	-20 °C to +45 °C (-4 °F to +113 °F)
Electrical voltage	100 to 240 VAC (50/60 Hz)
Power consumption	60 Watts
Dimensions (D x W x H)	38 cm x 35 cm x 29 cm (15” x 14” x 11.5”)
Net weight	6.0 kg (13.3 lbs.)
Shipping weight	11.4 kg (25 lbs.)
Warranty	One-year limited warranty on workmanship and parts

Subject to change
Accuracy and precision (within run) specifications apply to Advanced Instruments standards
and reference solutions. Performance at Reference Conditions: 20 °C to 25 °C (68 °F to 77 °F);
40 to 60% relative humidity
Operating Conditions: Temperature 18 °C to 35 °C (64 °F to 95 °F); 5 to 80% relative humidity
(non-condensing)
Dimensions when Micro-Sample Test Kit is on the instrument

Osmometer Calibration Standards and Reference Solutions

SK-OsmoTECH	Convenience Kit (Includes: TECH250, 3MA005, 3MA029, 3MA085, 3MA200, 3LA028, 3MA000)	View
3MA029	Clinitrol™ 290 Reference Solution, 10×2 mL ampules	View
3MA000	0 mOsm/kg H₂O Calibration Standard, 10×2 mL ampules	View
3MA005	50 mOsm/kg H₂O Calibration Standard, 10×2 mL ampules	View
3MA085	850 mOsm/kg H₂O Calibration Standard, 10×2 mL ampules	View
3MA200	2000 mOsm/kg H₂O Calibration Standard, 10×2 mL ampules	View
3LA028	Osmolality Linearity Set 100-2,000 mOsm 5x2x5 mL	View
3MA002	Osmolality Linearity Set, 0-2,000 mOsm 5x2x2 mL	View

Supplies

TECH250	Micro-Sample Test Kit: 1 boxes of 250 chamber cleaners, 250 sampler tips, 1 plunger wire	View
3M0828A	Plunger Wire, 1 per order (Replaces 3M0828- Plunger Wires, set of 2. Please note: A plunger wire is included in every 133800, TECH250, and XT100 Micro-Sample Test Kit box.)	View
3M0825	Ease-Eject 20-Microliter Sampler	View
240821	Sampler Tips, 50/pkg	View

Accessories

135022_EU	Epson Dot Matrix Printer (sold-separately). For CE marked printers - For all of EU & UK only	View
AN2TP5	Dot Matrix Printer Paper	View

Clinitrol™ 290 Reference Solution

10 x 2 mL Ampules

View Product

Osmometer Calibration Standards

Available from 0-4000 mOsm/kg H₂O 10 x 2 mL Ampules

View Product

Osmolality Linearity Set

0, 500, 900, 1500, 2000 mOsm/kg H₂O 5x 2x 2 mL Ampules

View Product

Protinol™ Protein-Based Controls

Protinol 3-Level Osmometer Control 3x3x3 mL

View Product

Biotech Services

Get the best performance from your osmometer from the people who designed and built it. We provide a range of services and service plans to meet the different needs of our customers. Our certified Field Service Engineers provide on-site professional support for the installation, training, validation, and maintenance of your osmometers.

Learn more

Protein Therapies

When it comes to process development and manufacturing of protein therapeutics, whether you’re making an antibody-based or other protein therapeutic, what you don’t know can slow you down.

Are all of your buffers and media formulated correctly?
Does your solution have unexpected components?
Are all of the steps involving solutions–i.e. cell growth, dialysis, chromatography–proceeding consistently and as expected?

With our innovative osmometers, you can gain valuable data for managing process and product quality through a simple 90-second test. Osmolality, the measurement of total solute concentration, is a reliable control specification and in-process parameter that can deliver information across the protein therapeutic process development and manufacturing workflow.

See all of our Osmometers

Application Notes

References

Amaral AI et al. Improving retroviral vectors production: Role of carbon sources in lipid biosynthesis. J Biotechnol. 2008 Nov 25;138(3-4):57-66.
Burger C et al. Systemic Mannitol-Induced Hyperosmolality Amplifies rAAV2-Mediated Striatal Transduction to a Greater Extent Than Local Co-infusion. Mol Ther. 2005 Feb;11(2):327-31.
Ferreira TB et al. Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production. Biotechnol Lett. 2005 Nov;27(22):1809-13.
Rodrigues AF et al. Production of Retroviral and Lentiviral Gene Therapy Vectors: Challenges in the Manufacturing of Lipid Enveloped Virus. In Viral Gene Therapy, ed. Ke Xu. London:IntechOpen Limited. 2011.
Cordova A et al. Osmotic Shock and the Strength of Viral Capsids. Biophys J. 2003 Jul;85(1):70-4.
Coroadinha AS. Effect of Osmotic Pressure on the Production of Retroviral Vectors: Enhancement in Vector Stability. Biotechnol Bioeng. 2006 Jun 5;94(2):322-9.

Cell Therapies

When the therapeutic you’re developing is cell-based, constant monitoring to optimize growth and ensure consistent culture conditions throughout multiple stages is critical for success.

Are your stem cells at highest viability and product quality levels throughout culture and passaging?
Are you ensuring a high rate of cell recovery post-thaw?
Are the methods and processes used for transduction optimized for your system?

Using osmolality can help your group answer these questions early in the process, ultimately helping you save time, money, and limited or costly reagents. Adding a simple 90-second osmolality measurement to the in-process parameters you measure is a quick and easy way to ensure optimal cell growth, during process development and manufacturing as well as during buffer and media preparation.

With our innovative osmometers, you can keep delicate cells at optimal growth and gain valuable data for managing process and product quality across the cell therapy process development and manufacturing workflow.

See all of our Bioprocessing Osmometers

Application Notes

References

Casula E et al. Osmotic behaviour of human mesenchymal stem cells: Implications for cryopreservation. PLoS One. 2017 Sep 8;12(9):e0184180.
Li Y and Ma T. Bioprocessing of Cryopreservation for Large-Scale Banking of Human Pluripotent Stem Cells. Biores Open Access. 2012 Oct;1(5):205-14.
Petrenko Y et al. Clinically Relevant Solution for the Hypothermic Storage and Transportation of Human Multipotent Mesenchymal Stromal Cells. Stem Cells Int. 2019 Jan 20;2019:5909524.
Rafiq QA et al. The role of biopreservation in cell and gene therapy bioprocessing. Cell & Gene Therapy Insights. 2017. ISSN: 2059-7800. 335-344.
Bohnenkamp H et al. Bioprocess development for the cultivation of human T-lymphocytes in a clinical scale. Cytotechnology. 2002 Jan;38(1-3):135-45.
Nie Y et al. Scalable Passaging of Adherent Human Pluripotent Stem Cells. PLoS One. 2014 Jan 30;9(1):e88012.
Potočar U et al. Adipose-Derived Stem Cells Respond to Increased Osmolarities. PLoS One. 2016 Oct 5;11(10):e0163870.

Gene Therapies

Consistency is critical when producing genetic therapies, including viral vector delivery and gene editing. Small deviations in the composition of cell growth media can have unwanted effects on cell density, cell composition, and virus yield.

Are you looking for ways to increase vector stability?
Is your gene therapy group looking for higher transfection efficiency and higher viral vector production?

Using osmolality can help your group work towards those goals, as well as answer:

- how to optimize the physiological range for transfection complexes
- identifying media needs
- determining effects of osmotic pressure on the stability of a complex

With our innovative osmometers, you can gain valuable data for managing process and product quality through a simple 90-second test. When done right, osmolality, the measurement of total solute concentration, is a reliable control specification and in-process parameter that can deliver information across the gene therapy process development and manufacturing workflow.

See all of our Osmometers

Application Notes

References

Amaral AI et al. Improving retroviral vectors production: Role of carbon sources in lipid biosynthesis. J Biotechnol. 2008 Nov 25;138(3-4):57-66.
Burger C et al. Systemic Mannitol-Induced Hyperosmolality Amplifies rAAV2-Mediated Striatal Transduction to a Greater Extent Than Local Co-infusion. Mol Ther. 2005 Feb;11(2):327-31.
Ferreira TB et al. Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production. Biotechnol Lett. 2005 Nov;27(22):1809-13.
Rodrigues AF et al. Production of Retroviral and Lentiviral Gene Therapy Vectors: Challenges in the Manufacturing of Lipid Enveloped Virus. In Viral Gene Therapy, ed. Ke Xu. London:IntechOpen Limited. 2011.
Cordova A et al. Osmotic Shock and the Strength of Viral Capsids. Biophys J. 2003 Jul;85(1):70-4.
Coroadinha AS. Effect of Osmotic Pressure on the Production of Retroviral Vectors: Enhancement in Vector Stability. Biotechnol Bioeng. 2006 Jun 5;94(2):322-9.

Personal Care

Osmolality testing has unlimited applications in areas of personal care and hygiene. Products that come into contact with or penetrate the skin must have an osmolality within a physiological range to avoid damaging cells and tissues. In some instances, slightly hypo- or hyper-osmotic solutions are generated to promote wetting or drying effects. The manufacturing of skincare products, personal lubricants and optical products

One key to a youthful look is healthy and moisturized skin. Proper hydration of skin depends on a balance of water flow into and out of skin cells and, thus, depends on osmolality. Osmolality testing of skincare products provides assurance that the product will have moisturizing effects and serves as a control during the production process.

The osmolality of personal lubricant formulations impacts their effect and safety. The World Health Organization (WHO) stated that the osmolality of these solutions should not exceed 1200 mOsm/kg, with this limit decreasing to a more iso-osmotic level in the future.

Similar value for osmolality testing can be clearly seen in optical applications. Eye drops and other optical products are designed to lubricate or protect the eye. The goal of these solutions (e.g. adding moisture, removing moisture, etc.) dictates an ideal osmolality range.

See all of our Osmometers

References

Aragona P et al. Physiochemical Properties of Hyaluronic Acid-Based Lubricant Eye Drops. Trans Vis Sci Tech. 2019;8(6):2.
Corrales R et al. Effects of Osmoprotectants on Hyperosmolar Stress in Cultured Human Corneal Epithelial Cells. Cornea 2008;27:574-579.
WHO/UNFPA/FHI, 2011-. Advisory Committee Notes to the Female Condom Technical Review Committee Meeting. [Geneva, Switzerland].

Vaccines

Osmolality testing is required to ensure that the final vaccine product is safe for administration. The osmolality must be within a physiological range to be introduced in human tissues. There is also evidence that injectable formulations with high osmolality may cause pain at the site of injection.

Vaccines are derived from a variety of sources, both living and synthetic, meaning a variety of bioproduction workflows are required. These workflows include some common use cases for osmolality testing, including the following:

Upstream cell culture and/or cell expansion requires maintaining an environment at the appropriate osmolality for the specific cell type. For those modalities that are cell-based, regular osmolality testing will ensure that the media and environment are optimized.
Buffers used during any downstream processing should be tested for osmolality to verify the concentrations of the solutions that are meant to protect the drug substance throughout the chromatography and/or filtration operations.
Final product testing must include osmolality to verify identify and safety of any injectable.

Differentiation in use cases for osmolality testing are outlined below.

Differentiation in use cases for osmolality testing are outlined below:

Monoclonal Antibody Vaccines

Osmolality testing should be performed on hybridoma cell media to ensure optimal antibody production rates. The rate of antibody production in hybridoma cells depends on the osmolality of the medium in which the cells are grown. The osmotic agent used to affect the osmolality levels also impacts Ab production.

Osmolality testing should be performed on hybridoma cell media to ensure optimal antibody production rates.
The rate of antibody production in hybridoma cells depends on the osmolality of the medium in which the cells are grown.
The osmotic agent used to affect the osmolality levels also impacts Ab production.

Viral Vaccines

Osmolality testing should be performed on media solutions to optimize cell size and viral vector stability. Osmotic pressure affects the size of the morphology of insect cells and viruses. The osmolality of the media affects the composition of the lipid bilayer membrane of enveloped viruses. This directly impacts the vector stability.

Osmolality testing should be performed on media solutions to optimize cell size and viral vector stability.
Osmotic pressure affects the size of the morphology of insect cells and viruses.
The osmolality of the media affects the composition of the lipid bilayer membrane of enveloped viruses. This directly impacts the vector stability.

mRNA and DNA Vaccines

Delivery systems and mechanisms should be tested for osmolality. mRNA can be complexed with different agents (e.g. PEI) for delivery within the body. Osmolality impacts the stability of the complex of viral models. Osmolality impacts the ability to penetrate the target cell membrane.

Delivery systems and mechanisms should be tested for osmolality.
mRNA can be complexed with different agents (e.g. PEI) for delivery within the body. Osmolality impacts the stability of the complex of viral models.
Osmolality impacts the ability to penetrate the target cell membrane.

See all of Our Osmometers

References

Hasan et al. Osmolytes in vaccine production, flocculation and storage: a critical review. I. 2019; 15(2): 514–525.
Coroadinha, AS et al. Effect of osmotic pressure on the production of retroviral vectors: Enhancement in vector stability. Biotechnol Bioeng 2006; 94(2):322-9.
Tanja A. Grein, Tobias Weidner and Peter Czermak. Concepts for the Production of Viruses and Viral Vectors in Cell Cultures. New Insights into Cell Culture Technology, pp.173-191.

Pardi, N., Hogan, M., Porter, F. et al. mRNA vaccines — a new era in vaccinology. Nat Rev Drug Discov 17, 261–279 (2018).
Choi H-J, Song J-M, Bondy BJ, Compans, RW, Kang S-M, Prausnitz MR (2015). Effect of Osmotic Pressure on the Stability of Whole Inactivated Influenza Vaccine for Coating on Microneedles. PLoS ONE 10(7): e0134431.
Rosinski, M. Osmolarity effects on observed insect cell size after baculovirus infection are avoided using growth medium for sample dilution. Biotechnol Prog. 2000 Sep-Oct;16(5):782-5.
Sonia Gutiérrez-Granados, Laura Cervera, Amine A. Kamen & Francesc Gòdia (2018): Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics, Critical Reviews in Biotechnology.

Oligonucleotides

This novel field involves the use of antisense oligonucleotides (ASOs), aptamers, siRNAs, miRNAs and synthetic mRNAs to control the expression of genes that have been associated with diseases. Multiple Advanced Instruments customers within this field use osmometers to verify the concentration of the solutions carrying their precious products. Ultimately, the quality of the drug is dependent on the process controls implemented throughout the workflow. Below are some of the current uses for osmolality testing in this field:

Buffer QC

Osmolality specifications for buffers to preserve and protect the critical quality attributes of the product as it is being developed.

Final Product

Compendial release test needed to confirm the identity and concentration of the oligo product for safe use.

Because this is an emerging field, it comes with a number of development and application challenges:

Delivery of RNAs across the lipid bilayer and into cells
Immunogenicity once in the body
High rates of degradation of oligos by plasma and tissues

Drug delivery into the target cells requires the proper osmotic pressure, similar to the parameters around viral transfection in vector development. The optimal osmolality of the delivery complex (e.g. mRNA:PEI) should be determined and monitored during this process. This specification will provide repeatable successful delivery of the drug.

See all of our Osmometers

References

Pardi, N., Hogan, M., Porter, F. et al. mRNA vaccines — a new era in vaccinology. Nat Rev Drug Discov 17, 261–279 (2018)
Poveda C, Biter AB, Bottazzi ME, Strych U. Establishing Preferred Product Characterization for the Evaluation of RNA Vaccine Antigens. Vaccines (Basel). 2019 Sep 27;7(4). pii: E131.

All models support Pharmacopeia testing guidelines	New! OsmoTECH^® HT Automated Micro-Osmometer	OsmoTECH^® PRO Multi-Sample Micro-Osmometer	OsmoTECH^® XT Single-Sample Micro-Osmometer	OsmoTECH^® Single-Sample Micro-Osmometer	Model 3250 Single-Sample Osmometer Being discontinued Dec. 31, 2020
Best for labs with daily sample processing needs of:	>20/day	>10/day	1-10/day	1-10/day	1-10/day
Sample volume	40 µL	30 µL	20 µL	20 µL	250 µL
Sample introduction	96 well plate	Sample cup	One-step direct	One-step direct	Sample cup
Operating range (mOsm/kg H₂O)	0 - 2,000 mOsm/kg H₂O	0 - 2,000 mOsm/kg H₂O	0 – 4,000 mOsm/kg H₂O	0 - 2,000 mOsm/kg H₂O	0 - 4,000
Test time (seconds)	140	90	Less than 150 in low range, Less than 190 in high range	90	120
Multi-sample capacity
Automated cleaning between samples
Complex Matrices (High Concentrations, Sugars, & Proteins)
Integrated barcode scanner
Onboard Video Instructions
Multi-language touchscreen display
Supports 21 CFR Part 11 & EU Annex 11 compliance
Electronic Signature (Review & Approve)
Supports Pharmacopeia testing guidelines compliance
Username & Password Compatibility (With Active Directory)
Dot-matrix Printer
Network Connection
Network share
Automatic backup
Control device remotely via OPC-UA
USB
Webserver for remote data viewing
Data Storage Capacity	Unlimited	Unlimited	Unlimited	Unlimited
Levels of users access	3 Levels	3 Levels	3 Levels	3 Levels
Data Format	.csv & pdf	.csv & pdf	.csv & pdf	.csv & pdf
Part #	OSMOTECHHT	OsmoTECHPRO	OsmoTECHXT	OsmoTECH	3250
Download Chart as a PDF Sold separately	Learn More Request Pricing	Learn More Request Pricing	Learn More Request Pricing	Learn More Request Pricing	Learn More Request Pricing

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Case Study

Osmolality: A Powerful Tool In Bioprocessing

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Application Note

Osmolality Monitoring For UF/DF And CEX Capture Of MAbs From Mammalian Cell Cultures

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Webinar

Tips & Tricks To Get The Most Out Of Your Osmometer

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Want to see the OsmoTECH live in action?

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What our customers say

This [OsmoTECH] instrument was a dream come true. The administrator can lock down features and dictate what users can and cannot do. Very easy to use and extremely reliable instrument.

Troy Franz

Paragon Bioservices

The [OsmoTECH] is highly effective and obtained results are of high quality. With this instrument I can develop my formulation for meeting the osmolality criteria for drug products.

Siva Bingi

Intas Pharmaceuticals

The best part of the [OsmoTECH] instrument is it gives accurate and reproducible results for any solution and most importantly in less than a minute you have the results.

Brijeshkumar Pandya

Nivagen Pharmaceuticals Inc.

[The OsmoTECH] achieves high quality and reproducible results. Easy instrument to use and well-presented software.

Claudia Chadford

Touchlight Genetics

[The OsmoTECH is] easy to use and robust piece of equipment that met all our user and regulatory requirements. The hardware is sleek, efficient and easy to use. The integrated software has built in data integrity controls and is thoroughly tested through validation test scripts / reports.

J. Lacey

Replimune

Request Pricing

For a quotation, to arrange a demonstration, or for more information please contact us by phone or complete the form.

Phone: +1-781-320-9000
Toll free: 800-225-4034 (US & Canada)

Austria: +49 695 0606 800
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Please fill out this brief form to download this content. You can also get in touch with our sales team for product questions, arranging a product demonstration or obtaining a quotation at the phone numbers below.

Phone: +1-781-320-9000
Toll free: 800-225-4034 (US & Canada)

Austria: +49 695 0606 800
Belgium: +31 6 899 10929
France: +33 456 681 861
Germany: +49 695 0606 800
The Netherlands: +31 6 899 10929
United Kingdom: +44 140 321 0400

China: +86 21 3412 6167

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For a quotation, to arrange a demonstration, or for more information please contact us by phone or complete the form.

Phone: +1-781-320-9000
Toll free: 800-225-4034 (US & Canada)

Austria: +49 695 0606 800
Belgium: +31 6 899 10929
France: +33 456 681 861
Germany: +49 695 0606 800
The Netherlands: +31 6 899 10929
United Kingdom: +44 140 321 0400

China: +86 21 3412 6167

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For more information about our trade in program please contact us by phone or complete the form.

Phone: +1-781-320-9000
Toll free: 800-225-4034 (US & Canada)

Austria: +49 695 0606 800
Belgium: +31 6 899 10929
France: +33 456 681 861
Germany: +49 695 0606 800
The Netherlands: +31 6 899 10929
United Kingdom: +44 140 321 0400

China: +86 21 3412 6167

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Compliance & Conformity

Compliance & Conformity