Lab Report 7: Facilitating Assay Transfer by Controlling Liquid Handling Variables

Assay transfer is one of the most complex activities in laboratories today.

Successful assay transfer ensures that new results are comparable to historical data. Whether it involves scaling an assay up to a higher throughput platform or transferring an assay from development to a QC environment, assay transfer is challenging because of the large number of variables involved. Differences in equipment, reagents, technique and interpretation of methodology are typically the focus of preparation before assay transfer. Less obvious variables, such as those that involve liquid handling, are often the cause of assay transfer failures. These frequently ignored variables are difficult to identify and, therefore, to troubleshoot.

Liquid handling instruments, such as pipettes and automated liquid handlers, are routinely used to perform assays in laboratories. Their accuracy is often taken for granted and, therefore, not given proper attention during assay transfer work. Understanding the importance that liquid handling plays in assay transfer work and taking measures to control liquid handling variables will ultimately facilitate assay transfer.