Osmolality as a concentration measurement method for key buffers in bioprocessing

This study compared osmolality to pH and conductivity across a wide range of concentrations of common downstream buffers. The buffer solutions were prepared gravimetrically and tested using a freezing point osmometer, pH probe and conductivity probe. The study showed that, for many solutions, osmolality is a reliable and sensitive measure of solute concentration compared to pH and conductivity. Together these process controls will help ensure optimal product quality and yield.