GloCyte® Automated Cell Counter for CSF

How MercyOne improved their cell count turnaround time by 30 minutes

Accurate, STAT cerebrospinal fluid (CSF) cell counts are critical in the diagnosis of meningitis, malignancy, demyelinating disease, and hemorrhage. Due to historical limitations with the linearity of automated analyzers, CSF cell counts have traditionally been performed manually with a hemocytometer and the aid of a brightfield optical microscope. Manual cell counting is time-consuming, subjective due to reliance on human interpretation, and requires a high degree of skill. This poses significant challenges for clinical labs of all sizes facing unprecedented staffing shortages.

The purpose of this study was to evaluate the impact of implementing the GloCyte Automated Cell Counter for CSF, an FDA-cleared device with linearity down to 0 cells/µL, on result turnaround times (TAT).

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How it Works

The GloCyte uses fluorescent microscopy and two reagents to enumerate total nucleated cells (TNCs) and red blood cells (RBCs) in under 5 minutes. A nucleic dye specifically stains the nucleus of TNCs and an antibody stain tags antigens on the surface of RBCs making it easy for techs of all skills levels to obtain accurate results. Stained samples are deposited onto test cartridges and vacuum is applied to capture cells on a membrane. An image of the membrane is captured by a digital CCD camera and processed via a sophisticated algorithm eliminating errors associated with manual counting.

What Our Customers Say

It is important for patients because it aids in the diagnosis of CSF involvement in hematologic malignancies. It prompts a diagnosis and early treatment, which always results in more favorable outcomes for the patient.

Alexander Macphie

University Hospitals Bristol and Weston NHS Foundation Trust

The [GloCyte] is easy to operate and user friendly. Quick and accurate results. [...] Steps are easy to remember. Counts RBC’s less than 1000 [...]. Easier to use than using the hemocytometer. NO MORE extra steps seen on using chamber counts: cleaning counting chamber and cover slip before counting, placing chamber in a moist box to wait for cells to settle, lysing RBC with acetic acid before WBC can be counted, focusing microscope etc.

Rodel Rosales

Boston Children's Hospital Core Laboratory

Case Study

This study conducted by Mercy One evaluates the impact of implementing the GloCyte Automated Cell Counter for CSF, an FDA-cleared device with linearity down to 0 cells/µL, on result turnaround times (TAT).

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