Reagents Help HEK Cell Line Viability And Viable Cell Density (VCD) During Cryopreservation And Revival

Cells often need a little extra support when it comes to stressful processes, such as cryopreservation and revival. Here we have a cell banking study which was conducted using ACF InstiTHAW HEK.

Cells were cryopreserved and revived with medium with and without InstiTHAW HEK to determine the supplements’ ability to support the viability and viable cell density (VCD) of cells.

Method

FreeStyle™ 293-F cells were cryopreserved at 1×10⁶ cells/mL under the following conditions:

• FreeStyle 293 expression medium supplemented with 10% DMSO, and InstiTHAW HEK.
• FreeStyle 293 expression medium supplemented with 10% DMSO.

Following cryopreservation, the cells were revived under the following conditions:

• FreeStyle 293 expression medium supplemented with InstiTHAW HEK.
• FreeStyle 293 expression medium.

The viability (%) and VCD (cells/mL) of the cells cryopreserved and revived under the various conditions were assessed via Trypan Blue exclusion on day 6 post-revival.