Reagents Help Clones Survive The Expansion Process And Boost Their Peak Viable Cell Density

In this case study we will examine a customer’s evaluation of the ACF InstiSHAKE CHO cell culture supplement. The customer’s objective was to reduce the number of clones lost during static to shaking expansion and improve the performance of their mAb expressing CHO-K1 cell line during fed-batch.

The project started using the VIPS™ instrument to automate the dispense of single cells into 96 well plates. BalanCD medium was supplemented with 5% InstiGRO CHO, in addition to the customer’s routine medium supplements. After 14 days culture, clones were assessed for IgG productivity (mg/L), and the top 96 clones were expanded to 24 well plates. To assess InstiSHAKE CHO’s ability to support clonal expansion, 48 of the 96 clones were expanded with InstiSHAKE CHO, while the remaining 48 were expanded using the customer’s standard protocol.